Since one of the characteristics of PNH is bone marrow failure and AA is frequently accompanied with hypoplastic or aplastic PIG-A mutation and lack of GPI-anchored proteins, and are frequently detectable in aplastic anemia (AA), and even in normal CD 55 −CD 59 − erythrocytes (PNH III erythrocytes) are consistently noted in paroxysmal nocturnal hemoglobinuria (PNH), characterized by Therefore, CD 55 −CD 59 − erythrocytes are profoundly susceptible to complement-mediated lysis. (GPI)-anchored proteins that inhibit the activation of complements on the cell membrane, thereby protecting cells against The CD55 (Decay Accelerating Factor) and CD59 (Membrane Inhibitor of Reactive Lysis) molecules are widely distributed glycosylphosphatidylinositol Previous Section Next Section Introduction May prove useful as a marker for PNH clone size. In differentiating PNH from AA patients with small numbers of PNH-phenotype erythrocytes, and the total reticulocyte count Comparative quantitative analysis of CD55 and CD59 molecules expressed on normal erythrocytes may be useful Total reticulocyte counts correlated strongly with the CD 55 −CD 59 − reticulocytes. Mean fluorescence level of CD 59 + mature erythrocytes was lowest in the PNH subjects (24.5 × 10 3 MESF), significantly less than in normal controls (39.0 × 10 3 MESF, p <0.05), and highest in the AA subjects (49.2 × 10 3 MESF). Mean fluorescence level of CD 55 + mature erythrocytes was lowest in the PNH patients (13.2 × 10 3 MESF), and was significantly lower than in normal controls (16.7 × 10 3 MESF, p <0.05), and highest in the AA subjects (22.8 × 10 3 MESF). Two-colorįlow cytometric analyses were conducted using thiazole orange, anti-CD55 and CD59 monoclonal antibodies to identify CD 55 −CD 59 − reticulocytes and mature erythrocytes. A total of 56 patients (AA 39 patients, PNH 17 patients) and 10 healthy volunteers were enrolled. Nocturnal hemoglobinuria (PNH), aplastic anemia (AA), and normal individuals in order to determine their usefulness for differentiationīetween PNH and AA. We quantified CD55 and CD59 molecules expressed on normal reticulocytes and mature erythrocytes of paroxysmal Since PNH occasionally results in bone marrow failure, it is difficult to differentiate PNH from AA with small numbers ofĬD 55 −CD 59 − erythrocytes.
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